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Keywords HBV X gene;eucaryotic expression;transactivating effect;SV40 early promoter; 乙肝病毒X基因;真核表达;反式激活;SV40早期启动子;
It is proved that the recombinated plasmid PUC18-X earring HBV X gene sequence. This experment will be a foundation for the following studies of HBx. 证实重组质粒PUC18-X含有HBV X基因编码序列。
Extracted DNAs were subjected to Alu-PCR or "Genome Walking" techniques to amplify the DNA segments containing the insertion sites between HBV X gene region and host genome. 用Alu-PCR技术或“Genome Walking”技术对所提取的组织DNA进行HBV X基因和侧方未知序列的PCR扩增。
Although it is not exactly understood how HBV induces HCC, with the development of molecular virology, the relationship between HCC and HBV X gene (HBx), and its protein pX was more and more revealed. 虽然HBV的确切致癌机制尚未完全阐明，但随着分子病毒学的研究进展，人们对HBVX基因(HBx)及其产物X蛋白(pX)与HCC之间的关系已有了多方面，多层次的了解，积累了大量研究资料。
HBV X gene HBVX基因
Result: The positive ratio of HBV DNA (X gene and/or S gene) is 73.1% in HCC group (49 of 67), 78.6% in CCC group (11 of 14), and 26.9% in normal control group (7 of 26). HBv DNA(x基因和/或S基因)阳性率:HCC组为73.;1%25(49例167例)，CCC组为78
X gene (HBx), enhancer II (Enh2) and basic core promoter (Bcp) of HBV in sera from HCC patients were amplified by nest PCR , and sequenced and then compared with the sequences in Genbank. 对HCC患者血清的HBV的HBx、Enh2与Bcp基因的巢式PCR产物直接测序，与Genbank中已发表序列进行对比分析。
Keywords HBV BCP;gene mutation;HCC; 基因变异;原发性肝癌;
Keywords Hepatitis B virus;Occult HBV infection;Cryptogenic hepatitis;X gene mutation; 肝炎病毒;乙型;不明原因性肝炎;隐匿性感染;基因变异;
Xiong J,Zi XY,Yao YC,et al.Establishment of transgenic mouse model habouring hepatitis B virus x gene[J].Di-er Junyi Daxue Xuebao(Acad J Sec Mil Med Univ),2002,23(11):1184-1187. [4]熊俊;訾晓渊;姚玉成;等.;乙型肝炎病毒x基因转基因小鼠模型的建立及培育[J]
Keywords HBV;Transgenic;Mouse embryo;Vitrification; 转基因;小鼠胚胎;玻璃化冷冻;
Keywords HBV;serologic markers;statistics; 血清学标志物;统计学;
Analysis of X gene and precore region mutations of hepatitis B virus in asymptomatic carrier and patients with chronic active hepatitis positive for anti-Hbe[J].Chin J Intern Med,2001,40(5): 335-6. HBe抗体阳性的无症状携带与慢性活动性肝炎的HBV X及前C基因变异[J].;中华内科杂志;2001;40(5):335-6
It is firstly characterized for the SCAN-(C2H2)X genes to truncate the zinc finger domains at the carboxyl terminus. 我们鉴定了SCAN-(C2H2)X基因会在其羧基端截除锌指结构域。
Keywords HBV;Examine;Epidemiology;Immunity inoculability; 乙型肝炎;监测;流行病学;免疫接种;
Keywords HBV bacterin;HBV surface antigen;Natural immune; 关键词乙肝疫苗;乙肝表面抗原;自然免疫;
Keywords HBV DNA;serologic markers;hepatic function; 乙型肝炎病毒DNA;血清学标志物;肝功能;
Keywords HBV;therapeutic peptide;epitope;molecular design;CTL; 表位;治疗性多肽;分子设计;CTL;
Keywords hepatitis B virus;X gene;deoxyribozyme;secondary structure; 乙型肝炎病毒;X基因;脱氧核酶;二级结构;
Keywords HBV DNA;transmission;mouse;oocyte;integration;chromosomes; 转染;小鼠;卵母细胞;整合;染色体;